Adsorption of Tannase from Aspergillus ficuum to carboxyl-functionalized multi-walled carbon nanotubes

The immobilization of cross-linked tannase onto carboxyl-functionalized multi-walled carbon nanotubes (MWCNT-COOH) was achieved via physical adsorption. Glutaraldehyde used to cross-link the enzyme molecules. Spectroscopic and morphological characterizations enzyme-nanotubes composite were carried out, which authenticated successful adsorption event. Enzyme is proven equal to, or even superior than free tannase, in terms catalytic activities stabilities, when measured under different thermal, pH recycling conditions. Whilst both immobilized preparations exhibited optimum catalysis at 5.0 35?C, tannase-nanotubes possesses better thermal stability. preparation retained 75 % its initial activity following ten consecutive uses. study demonstrated a facile method produce catalytically efficient nanobiocatalyst for biotechnological applications.